Application

Use Anti-Fluorescein for the detection of fluorescein-labeled compounds using:ELISAImmunohistocytochemistryIn situ hybridizationWestern blotAnti-Fluorescein has been used to capture amplicon in quadruple-tagging PCR and nucleic acid lateral flow assay for simultaneous detection of foodborne pathogens.

General description

Monoclonal antibody to fluorescein.The detection of bound antibody can be carried out directly in one step using an anti-mouse Ig fluorochrome/enzyme conjugate, or in a two-step procedure with anti-mouse Ig fluorescein and, subsequently, anti-fluorescein enzyme conjugate.The antibody does not contain any protein and can hence be used for coating and labeling.ContentsLyophilizate, stabilized

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline: •ELISA: 2 to 4 μg/ml•Immunohistocytochemistry: 0.5 to 2 μg/ml•In situ hybridization: 0.2 to 0.4 μg/ml•Western blot: 0.5 to 2 μg/mlWorking solution: Phosphate buffered saline, pH 7.4Using water instead the antibody can precipitate.

Reconstitution

Add 1 ml double-distilled water to a final concentration of 100 µg/ml.Reconstitute for 15 minutes.

Specificity

The monoclonal antibody reacts with free and bound fluorescein.